Vero cell assay for rapid detection of Clostridium perfringens enterotoxin
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چکیده
منابع مشابه
Vero cell assay for rapid detection of Clostridium perfringens enterotoxin.
A rapid assay which measured the biological activity of Clostridium perfringens enterotoxin was developed. The method involved the rapid killing of Vero cells by enterotoxin produced by C. perfringens grown in Duncan and Strong sporulation medium. Serial dilutions of toxin were added to Vero cells either in suspension or grown as monolayers in wells of a 96-well cell tissue culture cluster plat...
متن کاملRapid detection of Clostridium perfringens type A enterotoxin by enzyme-linked immunosorbent assay.
Clostridium perfringens type A enterotoxin was specifically detected and readily quantified by indirect and four-layer sandwich enzyme-linked immunosorbent assays (ELISAs). With the indirect ELISA, enterotoxin was detected in quantities of as low as 2.5 ng (25 ng/ml). When the more sensitive sandwich ELISA procedures was used, 100 pg (1 ng/ml) of enterotoxin was detected. The sandwich ELISA pro...
متن کاملEvaluation of ELISA, RPLA, and Vero cell assays for detecting Clostridium perfringens enterotoxin in faecal specimens.
Three hundred and ninety two faecal specimens from 70 separate outbreaks of suspected Clostridium perfringens food poisoning were examined by enzyme linked immunosorbent assay (ELISA), reversed passive latex agglutination (RPLA), and Vero cell assays for the presence of enterotoxin. Although the most time consuming method, ELISA was the most specific and reproducible. RPLA was slightly more sen...
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Three techniques for using the dog as an assay organism for Clostridium perfringens enterotoxin are described. These are believed to be more convenient than ligated ileal-loop procedures.
متن کاملAssay methods for Clostridium perfringens type A enterotoxin.
Enterotoxin produced by a sporulating culture of Clostridium perfringens type A NCTC 8798 was purified to a level of 3,500 mouse mean lethal doses per mg of nitrogen. High-titer sera were obtained from rabbits injected with enterotoxin and used to compare the sensitivity of serological tests and bioassays for C. perfringens enterotoxin. Reversed passive hemagglutination was by far the most sens...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1989
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.55.9.2141-2143.1989